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表面增强激光解吸/电离飞行时间(SELDI-TOF)质谱在确定唾液蛋白质组图谱中的应用。

Applications of Surface-Enhanced Laser Desorption/Ionization Time-Of-Flight (SELDI-TOF) Mass Spectrometry in Defining Salivary Proteomic Profiles.

作者信息

Al-Tarawneh Sandra K, Bencharit Sompop

机构信息

Department of Prosthodontics, School of Dentistry, University of North Carolina, Chapel Hill, NC, USA.

出版信息

Open Dent J. 2009 Apr 28;3:74-9. doi: 10.2174/1874210600903010074.

Abstract

Recent advancement in mass spectrometry leads us to a new era of proteomic analysis. Human saliva can be easily collected; however, the complexity of the salivary proteome in the past prevented the use of saliva for proteomic analysis. Here we review the development of proteomic analyses for human saliva and focus on the use of a new mass spectrometric technology known as surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF). SELDI-TOF, a modification of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF), combines the precision of mass spectrometry and the high-through-put nature of protein arrays known as Protein Chips. This technology shows a promising future for salivary proteomic analysis in monitoring treatments and diseases, as well as novel biomarker discovery.

摘要

质谱技术的最新进展引领我们进入了蛋白质组学分析的新时代。人类唾液易于采集;然而,过去唾液蛋白质组的复杂性阻碍了将唾液用于蛋白质组学分析。在此,我们回顾人类唾液蛋白质组学分析的发展,并重点关注一种称为表面增强激光解吸/电离飞行时间质谱(SELDI-TOF)的新型质谱技术的应用。SELDI-TOF是基质辅助激光解吸/电离质谱(MALDI-TOF)的一种改进,它结合了质谱的精确性和称为蛋白质芯片的蛋白质阵列的高通量特性。这项技术在监测治疗和疾病以及发现新型生物标志物方面的唾液蛋白质组学分析中显示出了广阔的前景。

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