Department of Automation, Shanghai Jiao Tong University, 800 Dongchuan Road, 200240, Shanghai, People's Republic of China.
Bioprocess Biosyst Eng. 2010 Aug;33(6):665-74. doi: 10.1007/s00449-009-0340-y. Epub 2009 Jun 19.
A macrokinetic model employing cybernetic methodology is proposed to describe mycelium growth and penicillin production. Based on the primordial and complete metabolic network of Penicillium chrysogenum found in the literature, the modeling procedure is guided by metabolic flux analysis and cybernetic modeling framework. The abstracted cybernetic model describes the transients of the consumption rates of the substrates, the assimilation rates of intermediates, the biomass growth rate, as well as the penicillin formation rate. Combined with the bioreactor model, these reaction rates are linked with the most important state variables, i.e., mycelium, substrate and product concentrations. Simplex method is used to estimate the sensitive parameters of the model. Finally, validation of the model is carried out with 20 batches of industrial-scale penicillin cultivation.
提出了一种采用控制论方法的宏观动力学模型来描述菌丝体生长和青霉素生产。该模型基于文献中发现的青霉素克鲁维酵母的原始完整代谢网络，通过代谢通量分析和控制论建模框架进行建模。抽象的控制论模型描述了基质消耗速率、中间产物同化速率、生物量生长速率以及青霉素形成速率的瞬态变化。结合生物反应器模型，这些反应速率与最重要的状态变量（即菌丝体、基质和产物浓度）相关联。单纯形法用于估计模型的敏感参数。最后，通过 20 批工业规模的青霉素发酵进行了模型验证。
