MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou, 510631, China.
Mol Imaging Biol. 2010 Jan-Feb;12(1):63-70. doi: 10.1007/s11307-009-0235-y. Epub 2009 Jun 19.
The aim of the study is to dynamically and non-invasively monitor the apoptosis events in vivo during photodynamic therapy (PDT) and chemotherapy.
A FRET probe, SCAT3, was utilized to determine activation of caspase-3 during tumor cell apoptosis in mice, induced by PDT, and cisplatin treatments. Using this method, dynamics of caspase-3 activation was observed both in vitro and in vivo.
Analysis of the fluorescent missions from tumor cells indicated that the caspase-3 activation started immediately after PDT treatment. In contrast, the caspase-3 activation started about 13 and 36 h after cisplatin treatment in vitro and in vivo, respectively.
FRET could be used effectively to monitor activation of caspase-3 in living organism. This method could be used to provide rapid assessment of apoptosis induced by anti-tumor therapies for improvement of treatment efficacy.
本研究旨在动态、无创地监测光动力疗法（PDT）和化学疗法过程中体内细胞凋亡事件。
采用荧光共振能量转移（FRET）探针 SCAT3 来检测 PDT 和顺铂处理诱导的小鼠肿瘤细胞凋亡过程中 caspase-3 的激活。利用该方法，我们在体外和体内观察到了 caspase-3 激活的动力学变化。
对肿瘤细胞的荧光发射分析表明，caspase-3 的激活在 PDT 治疗后立即开始。相比之下，caspase-3 的激活分别在体外和体内顺铂处理后 13 和 36 小时开始。
FRET 可有效用于监测活体内 caspase-3 的激活。该方法可用于快速评估抗肿瘤治疗引起的细胞凋亡，以提高治疗效果。
