Krüger Beate, Dandekar Thomas
Bioinformatik, Biozentrum Universitaet, Am Hubland, 97074 Universitaet Wuerzburg, Germany.
Methods Mol Biol. 2009;564:401-31. doi: 10.1007/978-1-60761-157-8_23.
Protein-protein interactions are the building blocks of cellular networks and at the heart of cellular regulation. However, their experimental identification is still a challenge. This chapter is concerned with the determination of protein-protein interactions by bioinformatical methods. These often can operate just on sequence information. Further required information is derived from public knowledge in literature databanks and biochemical databases as well as from the sequences themselves and iterative sequence comparisons. Further tools include domain analysis, structure prediction, and genome context methods. The results are predicted binary interactions and complete interaction networks.
蛋白质-蛋白质相互作用是细胞网络的构建基石，也是细胞调控的核心。然而，通过实验鉴定它们仍然是一项挑战。本章关注通过生物信息学方法确定蛋白质-蛋白质相互作用。这些方法通常仅基于序列信息进行操作。进一步所需的信息来自文献数据库和生化数据库中的公共知识，以及序列本身和迭代序列比较。其他工具包括结构域分析、结构预测和基因组上下文方法。结果是预测的二元相互作用和完整的相互作用网络。
