细胞色素P450花生四烯酸环氧化酶2J2在人肿瘤组织和细胞系中的表达。

Expression of cytochrome P450 arachidonic acid epoxygenase 2J2 in human tumor tissues and cell lines.

作者信息

Jiang Jian-Gang, Fu Xiang-Ning, Chen Chun-Lian, Wang Dao-Wen

机构信息

Department of Internal Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China.

出版信息

Ai Zheng. 2009 Feb;28(2):93-6. Epub 2009 Feb 23.

PMID:19550113

Abstract

BACKGROUND AND OBJECTIVE

Cytochrome P450 arachidonic acid epoxygenase 2J2 (CYP2J2) is a new metabolic pathway of arachidonic acid. However, its biological effects, especially pathophysiologic significance in human beings, remain to be further recognized. This study was to determine the expression of CYP2J2 in human tumor tissues and cell lines.

METHODS

The expression of CYP2J2 mRNA and protein in 130 specimens of human carcinoma and related adjacent normal tissues, four specimens of inflammatory pseudotumor tissues, eight human tumor cell lines and two normal cell lines (as control) was detected by reverse transcription-polymerase chain reaction (RT-PCR), western blot and immunohistochemistry.

RESULTS

CYP2J2 was highly expressed in 101 (78%) carcinoma tissues, but was not detected in adjacent normal tissues and inflammatory pseudo-tumor tissues. Its mRNA level was obviously correlated to its protein level (r = 0.613, p < 0.01). Immunohistochemistry analysis showed the same results as RT-PCR and western blot. Furthermore, CYP2J2 was only expressed in cancer cells but not in interstitial and inflammatory cells. CYP2J2 was highly expressed in all carcinoma cell lines, but not in two normal cell lines.

CONCLUSION

CYP2J2 is highly and selectively expressed in human tumor tissues and cell lines and may be a novel biomarker of human tumors.

摘要

背景与目的

细胞色素P450花生四烯酸环氧化酶2J2（CYP2J2）是花生四烯酸的一条新代谢途径。然而，其生物学效应，尤其是在人类中的病理生理意义，仍有待进一步认识。本研究旨在确定CYP2J2在人肿瘤组织和细胞系中的表达情况。

方法

采用逆转录-聚合酶链反应（RT-PCR）、蛋白质印迹法和免疫组织化学法检测130例人癌组织及相关癌旁正常组织、4例炎性假瘤组织、8种人肿瘤细胞系和2种正常细胞系（作为对照）中CYP2J2 mRNA和蛋白的表达。

结果

CYP2J2在101例（78%）癌组织中高表达，但在癌旁正常组织和炎性假瘤组织中未检测到。其mRNA水平与蛋白水平明显相关（r = 0.613，p < 0.01）。免疫组织化学分析结果与RT-PCR和蛋白质印迹法一致。此外，CYP2J2仅在癌细胞中表达，而在间质细胞和炎性细胞中不表达。CYP2J2在所有癌细胞系中高表达，但在两种正常细胞系中不表达。