二烯丙基二硫化物对人结肠癌SW480细胞周期阻滞的影响。

Effect of diallyl disulfide on cell cycle arrest of human colon cancer SW480 cells.

作者信息

Liao Qian-Jin, Su Jian, He Jie, Song Ying, Tang Hai-Lin, Su Qi

机构信息

Cancer Research Institute, The Second Affiliated Hospital, University of South China, Hengyang, Hunan, PR China.

出版信息

Ai Zheng. 2009 Feb;28(2):138-41. Epub 2009 Feb 15.

PMID:19550124

Abstract

BACKGROUND AND OBJECTIVE

Our previous study revealed that diallyl disulfide (DADS) significantly inhibited cell proliferation and induced cell cycle arrest at G(2)/M phase of human colon cancer SW480 cells. However, the molecular mechanism of cell cycle arrest remains unclear. This study was to investigate the role and the molecular mechanism of DADS in the induction of cell cycle arrest of human colon cancer cell line SW480.

METHODS

Proliferation of SW480 cells after DADS treatment was measured by MTT assay and cell counting. Phase distribution of cell cycle was analyzed by flow cytometry. Expressions of PCNA, p53, p21(WAF1) and cyclin B1 were detected by immunohistochemistry and western blot.

RESULTS

DADS (30-70 microg/mL) significantly inhibited proliferation and retarded the population doubling time of colonies in SW480 cells. Compared with the control group, SW480 cells were markedly accumulated at G(2)/M phase after the treatment with DADS (p < 0.05). Moreover, DADS remarkably decreased the protein contents of PCNA, p53 and cyclin B1, but increased the expression of p21(WAF1) in a time- and dose-dependent manner.

CONCLUSION

DADS induces G(2)/M arrest in human colon cancer SW480 cells, probably through the downregulation of PCNA, p53 and cyclin B1 and upregulation of p21(WAF1).

摘要

背景与目的

我们之前的研究表明，二烯丙基二硫化物（DADS）可显著抑制人结肠癌SW480细胞的增殖，并诱导细胞周期阻滞于G(2)/M期。然而，细胞周期阻滞的分子机制仍不清楚。本研究旨在探讨DADS在诱导人结肠癌细胞系SW480细胞周期阻滞中的作用及分子机制。

方法

采用MTT法和细胞计数法检测DADS处理后SW480细胞的增殖情况。通过流式细胞术分析细胞周期的阶段分布。采用免疫组织化学和蛋白质印迹法检测增殖细胞核抗原（PCNA）、p53、p21（WAF1）和细胞周期蛋白B1的表达。

结果

DADS（30 - 70μg/mL）显著抑制SW480细胞的增殖，并延长其集落群体倍增时间。与对照组相比，DADS处理后SW480细胞明显积聚于G(2)/M期（p < 0.05）。此外，DADS以时间和剂量依赖性方式显著降低PCNA、p53和细胞周期蛋白B1的蛋白含量，但增加p21（WAF1）的表达。