Liao Qian-Jin, Su Jian, Zhou Xiu-Tian, Tang Hai-Lin, Song Ying, Su Qi
Cancer Research Institute, University of South China, Hengyang, Hunan, 421001, PR China.
Ai Zheng. 2007 Aug;26(8):828-32.
BACKGROUND & OBJECTIVE: Diallyl disulfide (DADS) can inhibit the proliferation of various cancer cell lines in vitro, but little is known about its in vivo antitumor effect. This study was to investigate the inhibitory effect of DADS on the proliferation of human colon cancer cell line SW480 in nude mice.
After subcutaneous transplantation of SW480 cells in the back of nude mice, 5 mice received intraperitoneal injection of DADS (30 mg/kg), and 5 received intraperitoneal injection of normal saline as control. The body weight of nude mice and tumor growth were measured. The morphology of tumor was observed under optical microscope and electron microscope. The expression of proliferating cell nuclear antigen (PCNA) was detected by immunohistochemistry and morphometric quantitative analysis. Cell cycle distribution was analyzed by flow cytometry (FCM).
The growth of transplanted tumor was inhibited markedly by DADS; the relative tumor growth rate (T/C%) was 49.85%. In DADS group, the cellular atypia and nucleus-cytoplasm ratio were decreased, intracytoplasm cellular organs were abundant, and retrograde alters and apoptotic bodies were manifested in some cells. The protein level of PCNA was significantly lower in DADS group than in control group (149.02+/-4.26 vs. 178.86+/-7.69, P<0.05). SW480 cells in DADS group were arrested in G2/M phase; the G2/M phase proportion was significantly higher in DADS group than in control group (38.6% vs. 18.8%, P<0.01).
DADS has significant inhibitory effect on the proliferation of human colon cancer SW480 cells in nude mice and can arrest cell cycle in G2/M phase.
二烯丙基二硫醚（DADS）在体外可抑制多种癌细胞系的增殖，但其体内抗肿瘤作用尚不清楚。本研究旨在探讨DADS对人结肠癌SW480细胞株在裸鼠体内增殖的抑制作用。
将SW480细胞皮下移植于裸鼠背部后，5只裸鼠腹腔注射DADS（30mg/kg），5只裸鼠腹腔注射生理盐水作为对照。测量裸鼠体重和肿瘤生长情况。在光学显微镜和电子显微镜下观察肿瘤形态。采用免疫组织化学和形态计量学定量分析检测增殖细胞核抗原（PCNA）的表达。通过流式细胞术（FCM）分析细胞周期分布。
DADS显著抑制移植瘤生长；相对肿瘤生长率（T/C%）为49.85%。DADS组细胞异型性和核质比降低，胞质内细胞器丰富，部分细胞出现逆行改变和凋亡小体。DADS组PCNA蛋白水平显著低于对照组（149.02±4.26 vs. 178.86±7.69，P<0.05）。DADS组SW480细胞停滞于G2/M期；DADS组G2/M期比例显著高于对照组（38.6% vs. 18.8%，P<0.01）。
DADS对人结肠癌SW480细胞在裸鼠体内的增殖有显著抑制作用，并可使细胞周期停滞于G2/M期。
