Dragomir Nicoleta M, Goh Xiao M, Curl Claire L, Delbridge Lea M D, Roberts Ann
School of Physics, The University of Melbourne, Vic 3010, Australia.
Opt Express. 2007 Dec 24;15(26):17690-8. doi: 10.1364/oe.15.017690.
A novel application of quantitative phase imaging under linearly polarized light is introduced for studying unstained anisotropic live cells. The method is first validated as a technique for mapping the two-dimensional retardation distribution of a well-characterized optical fiber and is then applied to the characterization of unstained isolated cardiac cells. The experimental retardation measurements are in very good agreement with the established Brace-Köhler method, and additionally provide spatially resolved cell birefringence and phase data.
介绍了一种在线偏振光下定量相成像的新应用，用于研究未染色的各向异性活细胞。该方法首先作为一种绘制特性良好的光纤二维延迟分布的技术得到验证，然后应用于未染色的分离心肌细胞的表征。实验测得的延迟与既定的布雷斯-克勒方法非常吻合，并且还提供了空间分辨的细胞双折射和相位数据。
