Research Center on Aging, Sherbrooke, QC, Canada.
Atherosclerosis. 2009 Dec;207(2):420-7. doi: 10.1016/j.atherosclerosis.2009.05.017. Epub 2009 May 22.
Resveratrol, a polyphenolic constituent of red wine, is known for its anti-atherogenic properties and is thought to be beneficial in reducing the incidence of cardiovascular diseases (CVD). However, the mechanism of action by which it exerts its anti-atherogenic effect remains unclear. In this study, we investigated the relationship between the antioxidant effects of resveratrol and its ability to promote cholesterol efflux. We measured the formation of conjugated dienes and the rate of lipid peroxidation, and observed that resveratrol inhibited copper- and irradiation-induced LDL and HDL oxidation as observed by a reduction in oxidation rate and an increase in the lag phase (p<0.05). We used DPPH screening to measure free radical scavenging activity and observed that resveratrol (0-50microM) significantly reduced the content of free radicals (p<0.001). Respect to its effect on cholesterol homeostasis, resveratrol also enhanced apoA-1-mediated cholesterol efflux (r(2)=0.907, p<0.05, linear regression) by up-regulating ABCA-1 receptors, and reduced cholesterol influx or uptake in J774 macrophages (r(2)=0.89, p<0.05, linear regression). Incubation of macrophages (J774, THP-1 and MPM) with Fe/ascorbate ion, attenuated apoA-1 and HDL(3)-mediated cholesterol efflux whereas resveratrol (0-25microM) significantly redressed this attenuation in a dose-dependent manner (p<0.001). Resveratrol thus appears to be a natural antioxidant that enhances cholesterol efflux. These properties make it a potential natural antioxidant that could be used to prevent and treat CVD.
白藜芦醇是红酒中的一种多酚成分，具有抗动脉粥样硬化的特性，被认为有助于降低心血管疾病（CVD）的发病率。然而，其发挥抗动脉粥样硬化作用的机制尚不清楚。在这项研究中，我们研究了白藜芦醇的抗氧化作用与其促进胆固醇外排能力之间的关系。我们测量了共轭二烯的形成和脂质过氧化的速率，并观察到白藜芦醇抑制了铜离子和辐照诱导的 LDL 和 HDL 氧化，表现为氧化速率降低和滞后期延长（p<0.05）。我们使用 DPPH 筛选法来测量自由基清除活性，观察到白藜芦醇（0-50μM）显著降低了自由基含量（p<0.001）。关于其对胆固醇稳态的影响，白藜芦醇还通过上调 ABCA-1 受体增强了载脂蛋白 A-1 介导的胆固醇外排（r(2)=0.907，p<0.05，线性回归），并降低了 J774 巨噬细胞中的胆固醇内流或摄取（r(2)=0.89，p<0.05，线性回归）。用 Fe/抗坏血酸离子孵育巨噬细胞（J774、THP-1 和 MPM），减弱了载脂蛋白 A-1 和 HDL(3)介导的胆固醇外排，而白藜芦醇（0-25μM）以剂量依赖的方式显著纠正了这种减弱（p<0.001）。因此，白藜芦醇似乎是一种天然抗氧化剂，可增强胆固醇外排。这些特性使其成为一种潜在的天然抗氧化剂，可用于预防和治疗 CVD。
