妊娠弓形虫病：滤纸血样中 IgM、IgG 和亲和力的测定。
Toxoplasmosis in pregnancy: determination of IgM, IgG and avidity in filter paper-embedded blood.
机构信息
Instituto Nacional de Pediatría, Secretaría de Salud, México DF, México.
出版信息
J Perinatol. 2009 Oct;29(10):668-72. doi: 10.1038/jp.2009.79. Epub 2009 Jun 25.
OBJECTIVE
To detect immunoglobulin M (IgM) anti-Toxoplasma gondii antibodies and determine immunoglobulin G (IgG) titer and avidity in filter paper-embedded blood (FPEB) samples of pregnant women.
STUDY DESIGN
A total of 100 FPEB samples of pregnant women (30 positive and 70 negative) were analyzed for anti-T. gondii-specific IgM antibodies. Eleven and nine pairs of serum and FPEB samples were used to standardize IgG titration and avidity, respectively. Then, the correlation of avidity results was determined with 23 serum/FPEB pairs from IgG-positive cases.
RESULT
IgM detection in FPEB was 92% sensitive and 100% specific. The titration of IgG antibodies in FPEB correlated with that of serum (r >or=0.9). Significant difference in avidity between the acute and the undetermined/chronic cases was observed in both samples. As expected, no correlation was found between IgM levels and avidity.
CONCLUSION
The FPEB is useful to infer infection phase, and thus to speed clinical decisions in congenital toxoplasmosis management.
目的
检测孕妇滤纸包埋血样（FPEB）中的免疫球蛋白 M（IgM）抗弓形虫抗体，并确定免疫球蛋白 G（IgG）滴度和亲和力。
研究设计
共分析了 100 例孕妇的 FPEB 样本（30 例阳性和 70 例阴性），以检测抗弓形虫特异性 IgM 抗体。分别使用 11 对和 9 对血清和 FPEB 样本来标准化 IgG 滴度和亲和力。然后，用来自 IgG 阳性病例的 23 对血清/FPEB 对确定亲和力结果的相关性。
结果
FPEB 中 IgM 的检测灵敏度为 92%，特异性为 100%。FPEB 中 IgG 抗体的滴定与血清中 IgG 抗体的滴定相关（r≥0.9）。在两种样本中，急性和未确定/慢性病例的亲和力均存在显著差异。正如预期的那样，IgM 水平与亲和力之间没有相关性。
结论
FPEB 可用于推断感染阶段，从而加速先天性弓形虫病管理中的临床决策。
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