Liesenfeld O, Montoya J G, Kinney S, Press C, Remington J S
Department of Immunology and Infectious Diseases, Research Institute, Palo Alto Medical Foundation, Ames Bldg., 795 El Camino Real, Palo Alto, CA 94301, USA.
J Infect Dis. 2001 Apr 15;183(8):1248-53. doi: 10.1086/319672. Epub 2001 Mar 16.
The usefulness of testing for IgG avidity in association with Toxoplasma gondii was evaluated in a US reference laboratory. European investigators have reported that high-avidity IgG toxoplasma antibodies exclude acute infection in the preceding 3 months. In this US study, 125 serum samples taken from 125 pregnant women in the first trimester were chosen retrospectively, because either the IgM or differential agglutination (AC/HS) test in the Toxoplasma serologic profile suggested or was equivocal for a recently acquired infection. Of 93 (74.4%) serum samples with either positive or equivocal results in the IgM ELISA, 52 (55.9%) had high-avidity antibodies, which suggests that the infection probably was acquired before gestation. Of 87 (69.6%) serum samples with an acute or equivocal result in the AC/HS test, 35 (40.2%) had high-avidity antibodies. Forty women were given spiramycin, to prevent congenital transmission, and 7 (17.5%) had high-avidity antibodies. These findings highlight the value of testing a single serum sample obtained in the first trimester of pregnancy for IgG avidity.
美国一家参考实验室评估了检测弓形虫IgG亲和力的实用性。欧洲研究人员报告称，高亲和力的弓形虫IgG抗体可排除前3个月内的急性感染。在这项美国研究中，回顾性选取了125名孕早期孕妇的125份血清样本，因为弓形虫血清学检测中的IgM或差异凝集（AC/HS）试验提示或疑似近期获得性感染。在IgM ELISA检测结果为阳性或可疑的93份（74.4%）血清样本中，52份（55.9%）有高亲和力抗体，这表明感染可能发生在妊娠前。在AC/HS试验结果为急性或可疑的87份（69.6%）血清样本中，35份（40.2%）有高亲和力抗体。40名妇女接受了螺旋霉素治疗以预防先天性传播，其中7名（17.5%）有高亲和力抗体。这些发现凸显了检测孕早期采集的单一血清样本中IgG亲和力的价值。
