从西尼罗河病毒感染供体的血液中构建噬菌体抗体文库。
Construction of phage antibody repertoires from the blood of West Nile virus-infected donors.
作者信息
Throsby Mark, de Kruif John
机构信息
Crucell Holland BV, Archimedesweg 4, Leiden, The Netherlands.
出版信息
Methods Mol Biol. 2009;562:45-60. doi: 10.1007/978-1-60327-302-2_4.
PMID:19554286
Abstract
A method for the construction of West Nile virus immune donor antibody repertoires is described. B cells are harvested from a suitable donor and the antibody variable genes are amplified using polymerase chain reaction (PCR). The PCR fragments are cloned in a phage display vector to construct a repertoire that can be used in panning procedures to identify many unique monoclonal antibodies.
摘要
本文描述了一种构建西尼罗河病毒免疫供体抗体库的方法。从合适的供体中收获B细胞，并使用聚合酶链反应（PCR）扩增抗体可变基因。将PCR片段克隆到噬菌体展示载体中，以构建可用于淘选程序以鉴定许多独特单克隆抗体的抗体库。
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