Payne D, McLaughlin K J, Depypere H T, Kirby C A, Warnes G M, Matthews C D
Department of Obstetrics and Gynaecology, Queen Elizabeth Hospital, Woodville, South Australia.
Hum Reprod. 1991 Mar;6(3):423-31. doi: 10.1093/oxfordjournals.humrep.a137352.
Zona drilling (ZD) and zona cutting (ZC) were used in an IVF programme to assist fertilization in semen defect patients. Twenty-seven patients consented to ZD where acidified Tyrode's was used to create a hole in the zona pellucida. In 19 patients, ZD increased the fertilization rate to 29% compared with 8% (P less than 0.001) in their routine IVF cycles, and in eight patients precluded from routine IVF, a fertilization rate of 14% was achieved. Twenty-two patients consented to ZC where a slit in the zona is made mechanically. In 12 patients ZC increased the fertilization rate to 31% compared with 14% (P less than 0.01) from previous routine IVF cycles, and in 10 patients precluded from routine IVF, a fertilization rate of 34% was achieved. In 13 cycles, 68 uncut control oocytes were inseminated. In five cycles both control and ZC oocytes were fertilized (n.s.d.). In eight cycles no control oocytes were fertilized compared with 27% of ZC oocytes. The polyspermy rate was 4.6%. Twenty-four per cent of ZD and 12% of ZC (P less than 0.01) oocytes and embryos were degenerate after 42 h. Both ZD and ZC can increase the fertilization rate of sub-optimal semen, however, in our hands neither technique produced a pregnancy.
在一项体外受精（IVF）计划中，采用透明带钻孔（ZD）和透明带切割（ZC）技术来辅助精液缺陷患者的受精过程。27名患者同意进行ZD，使用酸化的台氏液在透明带上制造一个孔。在19名患者中，与常规IVF周期中的8%相比，ZD将受精率提高到了29%（P小于0.001），而在8名无法进行常规IVF的患者中，受精率达到了14%。22名患者同意进行ZC，通过机械方式在透明带上制造一个切口。在12名患者中，与之前常规IVF周期中的14%相比，ZC将受精率提高到了31%（P小于0.01），而在10名无法进行常规IVF的患者中，受精率达到了34%。在13个周期中，对68个未切割的对照卵母细胞进行了授精。在5个周期中，对照卵母细胞和ZC卵母细胞均受精（无显著差异）。在8个周期中，对照卵母细胞未受精，而ZC卵母细胞的受精率为27%。多精受精率为4.6%。42小时后，24%的ZD卵母细胞和胚胎以及12%的ZC卵母细胞和胚胎发生退化。ZD和ZC均可提高精液质量欠佳患者的受精率，然而，在我们的研究中，这两种技术均未成功实现妊娠。
