CDC25A和细胞周期蛋白E表达对原发性乳腺癌患者的预后影响

Prognostic implication of CDC25A and cyclin E expression on primary breast cancer patients.

作者信息

Mehdipour Parvin, Pirouzpanah Saeed, Sarafnejad Abdolfattah, Atri Morteza, Shahrestani S Tahereh, Haidari Mojgan

机构信息

Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Pour Sina Ave., Tehran 1417613151, IR Iran.

出版信息

Cell Biol Int. 2009 Oct;33(10):1050-6. doi: 10.1016/j.cellbi.2009.06.016. Epub 2009 Jun 23.

DOI:10.1016/j.cellbi.2009.06.016

PMID:19555767

Abstract

Defect in cell cycle control is a hallmark character of cancer. We have investigated the association of Ki67 labeling index, cyclin E and CDC25A expressions with clinical follow-up data in breast carcinomas. Flow cytometry was used to detect gene amplification of cyclins in 44 tumor tissue with invasive breast carcinomas. Multivariate Cox proportional hazard ratio test was used to show the correlations. Cyclin E or CDC25A were upregulated in 34% of the tumors. Among the whole total material, expression of cyclin E and of CDC25A were found upregulated in 31.9% and 39.4% of cells, respectively. Both CDC25A and cyclin E protein expression levels were correlated with Ki67 expression level (p<0.001). In addition, the expression of CDC25A was associated significantly with poor survival (P=0.028), whereas no correlation was found with cyclin E. These findings suggest a possible prognostic value for CDC25A as a cell cycle marker and may imply in characteristic of high risk breast cancer patients.

摘要

细胞周期调控缺陷是癌症的一个标志性特征。我们研究了乳腺癌中Ki67标记指数、细胞周期蛋白E和细胞周期蛋白依赖性激酶25A（CDC25A）表达与临床随访数据之间的关联。采用流式细胞术检测44例浸润性乳腺癌肿瘤组织中细胞周期蛋白的基因扩增情况。使用多变量Cox比例风险比检验来显示相关性。34%的肿瘤中细胞周期蛋白E或CDC25A上调。在整个材料中，分别有31.9%和39.4%的细胞中发现细胞周期蛋白E和CDC25A的表达上调。CDC25A和细胞周期蛋白E的蛋白表达水平均与Ki67表达水平相关（p<0.001）。此外，CDC25A的表达与较差的生存率显著相关（P=0.028），而与细胞周期蛋白E无相关性。这些发现表明CDC25A作为一种细胞周期标志物可能具有预后价值，并且可能暗示高危乳腺癌患者的特征。