Arnutti Pasra, Kotepui Manas, Asanprakit Wichitra, Punyarit Phaibul, Chavalitshewinkoon-Petmitr Porntip, Harnroongroj Talabporn, Petmitr Songsak
Department of Tropical Nutrition & Food Science, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand.
Int J Clin Exp Pathol. 2013 May 15;6(6):1112-20. Print 2013.
Breast cancer is the most common cancer affecting women worldwide including Thailand. Whole transcription profiles of invasive ductal breast carcinoma (IDC) obtained by oligonucleotide microarray should lead to a better understanding of the molecular basis of IDCs, allow for examination of specific markers for diagnosis, and provide novel targets for therapy. This study aimed to detect the whole transcript expression of approximately 35,000 target genes in Thai breast cancer patients, using Affymetrix GeneChip(®) Exon 1.0 Sense Target Arrays. Analysis revealed that the differential expression profiles of 928 genes (423 up-regulated and 505 down-regulated genes) were 2-fold or greater (unpaired t-test, p < 0.05) in invasive ductal breast cancer, compared with normal tissues. The Gene Ontology (GO) databases support important associations in 17 gene sets with p-value < 1E-10 and ≥ 4-fold changes, involving the tumorigenic pathways of cell cycles, extracellular regions, as well as cellular component organization. Likewise, the TGFBR and IL-6 pathways contain gene expression with statistically significant changes in IDC.
乳腺癌是包括泰国在内的全球女性中最常见的癌症。通过寡核苷酸微阵列获得的浸润性导管癌（IDC）的全转录谱，应能更好地理解IDC的分子基础，有助于检查诊断的特异性标志物，并为治疗提供新靶点。本研究旨在使用Affymetrix GeneChip(®) Exon 1.0 Sense Target Arrays检测泰国乳腺癌患者中约35000个靶基因的全转录表达。分析显示，与正常组织相比，浸润性导管癌中928个基因（423个上调基因和505个下调基因）的差异表达谱为2倍或更高（未配对t检验，p < 0.05）。基因本体论（GO）数据库支持17个基因集的重要关联，p值< 1E-10且变化≥ 4倍，涉及细胞周期、细胞外区域以及细胞成分组织的致瘤途径。同样，TGFBR和IL-6途径在IDC中包含具有统计学显著变化的基因表达。
