Canale M, Bistarini S, Merler M
Arch Toxicol. 1977 Jun 18;37(2):143-7. doi: 10.1007/BF00293864.
A new type of chromatographic column of silica gel 20 mm in diameter and 180 mm long has been investigated in the field of forensic analytical toxicology. In order to evaluate the usefulness of chromatobars in isolating organic compounds from biological extracts, sodium phenyl-ethyl-barbiturate, atropine, morphine and strychnine were added to the aqueous extract obtained with Stas-Otto's isolation method. Afterwards the residues of the acidic ether extract, alkaline ether extract and alkaline chloroform extract, were chromatographed on chromatobar. This chromatographic device seems able to avoid the drawbacks and limitations of paper and thin-layer chromatography, mainly from the preparative aspect. The method is particularly advantageous in analysing the whole extract obtained with the Stas-Otto process, without bringing about its partition, especially when slight traces of toxic substances are present.
一种直径20毫米、长180毫米的新型硅胶色谱柱已在法医分析毒理学领域得到研究。为了评估色谱棒在从生物提取物中分离有机化合物方面的实用性，将苯巴比妥钠、阿托品、吗啡和士的宁添加到用斯塔斯-奥托分离法获得的水提取物中。然后，将酸性乙醚提取物、碱性乙醚提取物和碱性氯仿提取物的残留物在色谱棒上进行色谱分析。这种色谱装置似乎能够避免纸色谱和薄层色谱的缺点和局限性，主要是在制备方面。该方法在分析用斯塔斯-奥托方法获得的整个提取物时特别有利，不会导致其分配，尤其是当存在微量有毒物质时。
