Preheim L C, Gentry M J, Snitily M U
Section of Infectious Diseases, Veterans Affairs Medical Center, Omaha, NE 68105.
J Infect Dis. 1991 Dec;164(6):1203-6. doi: 10.1093/infdis/164.6.1203.
Cirrhotic rats have decreased pulmonary bactericidal activity and increased bacteremia after experimental pneumococcal pneumonia. To determine if this finding is due to impaired pulmonary recruitment of polymorphonuclear leukocytes (PMNL), bronchoalveolar lavage (BAL) was done on cirrhotic and normal rats after transtracheal challenge with pneumococcal types 3 and 1. Mean absolute numbers of recruited PMNL in BAL fluid (BALF) at 2, 4, 6, 8, and 24 h after 10(7) cfu of type 3 challenge were similar in cirrhotic and normal rats. In both groups, lower numbers of PMNL were recruited after challenge with 10(5) cfu of type 3. Type 1 pneumococci stimulated recruitment of similar mean absolute numbers of PMNL (x10(7] in BALF (cirrhotics vs. normals) at 24 h after challenges with 10(5) cfu (0.3 +/- 0.1 vs. 0.3 +/- 0.1) and 10(7) cfu (2.9 +/- 1.3 vs. 2.8 +/- 0.7). Peripheral blood PMNL from cirrhotic and normal rats did not differ in adherence to nylon wool columns or in chemotaxis toward lipopolysaccharide-activated normal rat serum. Thus the impaired pulmonary defense against pneumococcal pneumonia in cirrhosis is not due to deficient pulmonary PMNL recruitment.
肝硬化大鼠在实验性肺炎球菌肺炎后肺杀菌活性降低且菌血症增加。为了确定这一发现是否归因于多形核白细胞（PMNL）向肺内募集受损，在用3型和1型肺炎球菌经气管攻击肝硬化大鼠和正常大鼠后进行支气管肺泡灌洗（BAL）。在给予10⁷cfu 3型肺炎球菌攻击后2、4、6、8和24小时，肝硬化大鼠和正常大鼠支气管肺泡灌洗液（BALF）中募集的PMNL平均绝对数量相似。在两组中，给予10⁵cfu 3型肺炎球菌攻击后募集的PMNL数量较少。在给予10⁵cfu（0.3±0.1对0.3±0.1）和10⁷cfu（2.9±1.3对2.8±0.7）攻击后24小时，1型肺炎球菌刺激BALF中募集的PMNL平均绝对数量相似（肝硬化大鼠对正常大鼠，单位为10⁷）。肝硬化大鼠和正常大鼠外周血PMNL在对尼龙毛柱的黏附或对脂多糖激活的正常大鼠血清的趋化性方面没有差异。因此，肝硬化时对肺炎球菌肺炎的肺防御受损并非由于肺内PMNL募集不足。
