Barber D, Parr S R, Greenwood C
Biochem J. 1977 Jun 1;163(3):629-32. doi: 10.1042/bj1630629.
The reduction of cytochrome c551 oxidase from Pseudomonas aeruginosa by Cr2+ ions was followed in the stopped-flow apparatus at a number of wavelengths. The c-haem reduction proceeded in a biphasic fashion with second-order rate constants of 2.6 X 10(5)M-1-S-1 and 4.8 X 10(4)M-1-S-1 at 25 degrees C, whereas the biphasic reduction of the d1-haem appeared to be independent of reductant concentration with rate constants of approx. 1.0S-1 and 0.25S-1 respectively. The kinetically determined difference spectra (reduced minus oxidized) for the c- and d1-haems are presented.
在停流装置中，于多个波长下跟踪了Cr2+离子对铜绿假单胞菌细胞色素c551氧化酶的还原过程。在25℃时，c-血红素的还原呈双相方式进行，二级速率常数分别为2.6×10(5)M-1·S-1和4.8×10(4)M-1·S-1，而d1-血红素的双相还原似乎与还原剂浓度无关，速率常数分别约为1.0S-1和0.25S-1。给出了通过动力学测定得到的c-和d1-血红素的差示光谱（还原态减去氧化态）。
Zotero 插件