Ren Lei, Jiang Zi Qiang, Fu Yun, Leung Wai Keung, Jin Lijian
Department of Periodontology, Institute of Stomatological Research, Guanghua School of Stomatology, Sun Yat-Sen University, Guangzhou, China.
J Clin Periodontol. 2009 Aug;36(8):619-26. doi: 10.1111/j.1600-051X.2009.01436.x. Epub 2009 Jun 26.
Periodontal pathogenesis is characterized by Gram-negative bacteria activation of series of pro- and anti-inflammatory cytokines from host cells through the pathway of lipopolysaccharide (LPS), LPS-binding protein (LBP) and CD14. The present study investigated the expression profiles of interleukin (IL)-1beta and IL-10 in periodontal health and disease, and examined the effects of Escherichia coli LPS and LBP interaction on the expression of IL-1beta and IL-10 by human gingival fibroblasts (HGF).
Gingival biopsies were collected from 44 subjects with chronic periodontitis and 15 periodontally healthy subjects. The expression of IL-1beta and IL-10 was detected by immunohistochemistry. The mRNA expression of IL-1beta and IL-10 in HGF was detected by RT-PCR with or without recombinant human LBP (rhLBP), while the peptides were analysed by an enzyme-linked immunosorbent assay.
IL-1beta was detected in both oral sulcular epithelia of healthy controls and periodontal pocket epithelia of patients. IL-10 was mainly expressed in the intercellular spaces of connective tissues. IL-1beta displayed a reverse pattern of expression levels with reference to IL-10, and a negative correlation existed between LBP and the ratio of IL-1beta/IL-10. rhLBP suppressed E. coli LPS-induced IL-1beta expression by HGF.
An appropriate interplay of LBP and cytokines may have a beneficial effect on innate host defence, thereby contributing to periodontal homeostasis.
牙周病发病机制的特征是革兰氏阴性菌通过脂多糖（LPS）、LPS结合蛋白（LBP）和CD14途径激活宿主细胞一系列促炎和抗炎细胞因子。本研究调查了白细胞介素（IL）-1β和IL-10在牙周健康和疾病中的表达谱，并检测了大肠杆菌LPS与LBP相互作用对人牙龈成纤维细胞（HGF）中IL-1β和IL-?10表达的影响。
从44例慢性牙周炎患者和15例牙周健康受试者中采集牙龈活检组织。采用免疫组织化学法检测IL-1β和IL-10的表达。在有或无重组人LBP（rhLBP）的情况下，通过逆转录聚合酶链反应（RT-PCR）检测HGF中IL-1β和IL-10的mRNA表达，同时采用酶联免疫吸附测定法分析其肽段。
在健康对照者的口腔龈沟上皮和患者的牙周袋上皮中均检测到IL-1β。IL-10主要表达于结缔组织的细胞间隙。IL-1β的表达水平与IL-10呈相反模式，且LBP与IL-1β/IL-10比值呈负相关。rhLBP抑制大肠杆菌LPS诱导的HGF中IL-1β表达。
LBP与细胞因子之间的适当相互作用可能对宿主天然防御产生有益影响，从而有助于牙周稳态。
