Sexton Tom, Bantignies Frédéric, Cavalli Giacomo
Institute of Human Genetics CNRS - UPR 1142, 141 Rue de la Cardonille, 34396 Montpellier, France.
Semin Cell Dev Biol. 2009 Sep;20(7):849-55. doi: 10.1016/j.semcdb.2009.06.004. Epub 2009 Jun 24.
The chromosome conformation capture (3C) technique and its genome-wide applications ('4C') have identified a plethora of distal DNA sequences that are frequently in close spatial proximity. In many cases, these have been correlated with transcriptional regulation of the interacting genes, but the functional significance of many of the extreme long-range and interchromosomal interactions remains unclear. This review summarises our current understanding of how chromatin conformation can impinge on gene expression, the major questions that need to be addressed to understand this more fully, and how these questions may be answered in the near future.
染色体构象捕获（3C）技术及其全基因组应用（“4C”）已鉴定出大量经常在空间上紧密相邻的远端DNA序列。在许多情况下，这些序列与相互作用基因的转录调控相关，但许多极远距离和染色体间相互作用的功能意义仍不清楚。本综述总结了我们目前对染色质构象如何影响基因表达的理解、为更全面理解这一过程需要解决的主要问题，以及这些问题在不久的将来可能如何得到解答。
