Albert-Ludwigs-Universität, Institut für Pharmazeutische Wissenschaften, Pharmazeutische Biologie und Biotechnologie, Freiburg, Germany.
Phytochemistry. 2009 Oct-Nov;70(15-16):1812-21. doi: 10.1016/j.phytochem.2009.05.019. Epub 2009 Jun 24.
The bioactivity of many natural products produced by microorganisms can be attributed to their sugar substituents. These substituents are transferred as nucleotide-activated sugars to an aglycon by glycosyltransferases. Engineering these enzymes can broaden their substrate specificity and can therefore have an impact on the bioactivity of the secondary metabolites. In this review we present the generation of a glycosyltransferase gene toolbox which contains more than 70 bacterial glycosyltransferases to date. Investigations of the function, specificity and structure of these glycosyltransferases help to understand the great potential of these enzymes for natural product biosynthesis.
许多微生物产生的天然产物的生物活性可以归因于它们的糖取代基。这些取代基被核苷酸激活的糖通过糖基转移酶转移到糖苷配基上。对这些酶进行工程改造可以拓宽它们的底物特异性，从而对次级代谢物的生物活性产生影响。在这篇综述中，我们介绍了迄今为止超过 70 种细菌糖基转移酶的基因工具盒的生成。对这些糖基转移酶的功能、特异性和结构的研究有助于理解这些酶在天然产物生物合成中的巨大潜力。
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