牙周组织形态计量学和去卵巢、营养不良及糖皮质激素应用后老年绵羊的状况。

Department of Oral Surgery, Medical University of Vienna, Währingerstrasse 25a, 1090 Vienna, Austria.

Arch Oral Biol. 2009 Sep;54(9):857-63. doi: 10.1016/j.archoralbio.2009.05.010. Epub 2009 Jun 26.

OBJECTIVE

Ageing, hypogonadism, malnutrition, and the application of glucocorticoids have adverse effects on skeletal homeostasis. Herein we determined to which extent the periodontium undergoes catabolic changes under these conditions in a sheep model.

METHODS

Six old sheep with a mean age of 7.5+/-1.0 years were subjected to ovariectomy, calcium/vitamin D-restricted diet, and intramuscular administration of approximately 2g methylprednisolone. Six adult sheep with a mean age of 3.8+/-0.9 years remained untreated and served as controls. First and second premolars of both jaws were subjected to histological analysis. The distances from the gingival margin (GM) and from the alveolar bone crest (ABC) to the cemento-enamel junction (CEJ) were determined. Periodontal attachment was given as the ratio between the dimension of the periodontal ligament and the alveolar bone. Clinical data were collected by counting the number of teeth missing, teeth with gingival recession, and teeth with a probing depth > 4 mm.

RESULTS

We report that distance between GM and CEJ (2.1+/-1.7 mm and 6.6+/-2.6mm maxilla; -0.4+/-1.4 mm and 3.2+/-1.5 mm mandible), and between ABC and CEJ (-3.4+/-1.3mm and 1.8+/-2.7 mm maxilla; -3.5+/-1.1mm and -0.1+/-1.4mm mandible) are significantly lower in test than in control animals. In line with these findings, periodontal attachment was 67% in the maxilla and 86% in the mandible of the test group and almost completely preserved in the control group. Clinical evaluation showed that the overall number of teeth with recessions was significantly higher in the test compared to the control group (4.9+/-2.4 and 2.3+/-3.6), but not the number of teeth missing and teeth with a probing depth>4mm.

CONCLUSIONS

Together these findings suggest that in sheep, the cumulating effects of ageing, hypogonadism, malnutrition and glucocorticoid application can cause substantial catabolic changes of the periodontium.

目的

衰老、性腺功能减退、营养不良和糖皮质激素的应用对骨骼稳态有不良影响。在此，我们在绵羊模型中确定了在这些条件下牙周组织会在多大程度上发生分解代谢变化。

方法

将 6 只平均年龄为 7.5+/-1.0 岁的老年绵羊进行卵巢切除术、钙/维生素 D 限制饮食和肌肉内注射约 2g 甲基强的松龙。6 只平均年龄为 3.8+/-0.9 岁的成年绵羊未接受治疗，作为对照组。对上下颌的第一和第二前磨牙进行组织学分析。从牙龈缘（GM）和牙槽骨嵴（ABC）到牙釉质牙骨质界（CEJ）的距离被确定。牙周附着被定义为牙周韧带和牙槽骨之间的尺寸比。通过计数缺失牙、牙龈退缩牙和探诊深度>4mm 的牙来收集临床数据。

结果

我们报告 GM 和 CEJ 之间的距离（上颌 2.1+/-1.7mm 和 6.6+/-2.6mm；下颌-0.4+/-1.4mm 和 3.2+/-1.5mm）以及 ABC 和 CEJ 之间的距离（上颌-3.4+/-1.3mm 和 1.8+/-2.7mm；下颌-3.5+/-1.1mm 和-0.1+/-1.4mm）在实验组中明显低于对照组。与这些发现一致，实验组上颌和下颌的牙周附着率分别为 67%和 86%，而对照组几乎完全保留。临床评估显示，实验组中牙龈退缩的牙齿总数明显高于对照组（4.9+/-2.4 和 2.3+/-3.6），但缺失牙和探诊深度>4mm 的牙齿数量没有差异。