Kobayashi S, Yamamoto J, Kaneda Y, Nishino K, Itokawa Y
Akita Prefectural Institute of Public Health.
Nihon Eiseigaku Zasshi. 1991 Aug;46(3):762-8. doi: 10.1265/jjh.46.762.
A method for determination of zinc contents in very small samples of hair was developed in order to estimate the zinc concentration in the human body. All steps from weighing of samples to ashing and preparation of the test solution were done in a tube designed by us to prevent contamination and to improve handling. Zinc concentration was determined with flame atomic absorption spectrophotometry aspirating a small volume of samples (80 microliters), by the so-called "one-drop method". Using this method, 5 mg of whiskers was analyzed every day for 10 days, and this revealed that the fluctuation of the zinc concentration level was very small and that it was possible to trace the individual level of zinc concentration daily. A sample of 5 mg is enough for the application of this method. Zinc concentration in the hair under the temple of a boy, and even the low-level zinc content of patients receiving total parenteral nutrition could be accurately determined every 10 days and monthly, respectively. The level of hair zinc contents for neonates was similar to that of adults.
为了估算人体中的锌浓度，开发了一种测定极少量头发样本中锌含量的方法。从样本称重到灰化以及测试溶液制备的所有步骤均在我们设计的试管中进行，以防止污染并便于操作。锌浓度通过火焰原子吸收分光光度法，采用所谓的“一滴法”，吸取少量样本（80微升）进行测定。使用该方法，每天分析5毫克胡须样本，持续10天，结果显示锌浓度水平的波动非常小，并且能够每日追踪个体的锌浓度水平。5毫克的样本量足以应用此方法。分别每10天和每月可以准确测定一名男孩太阳穴处头发的锌浓度，甚至是接受全胃肠外营养患者的低水平锌含量。新生儿的头发锌含量水平与成年人相似。
