哺乳动物细胞核中DNA复制位点的可视化。

Visualization of DNA replication sites in mammalian nuclei.

作者信息

Dimitrova Daniela S

机构信息

Chromatin and Gene Expression Department, The Babraham Institute, Cambridge, UK.

出版信息

Methods Mol Biol. 2009;521:413-36. doi: 10.1007/978-1-60327-815-7_23.

DOI:10.1007/978-1-60327-815-7_23

PMID:19563120

Abstract

DNA replication takes place at discrete sites in the cell nucleus, named replication foci. The spatial arrangements of these foci change in the course of S phase in a temporally regulated and reproducible fashion forming five distinct and highly conserved replication patterns. The organization of nuclear replication sites can be studied by electron and light microscopy techniques. This chapter describes several procedures for detection of replication foci in mammalian nuclei via indirect immunofluorescence microscopy.

摘要

DNA复制发生在细胞核内的离散位点，即复制灶。这些复制灶的空间排列在S期过程中以时间调控且可重复的方式发生变化，形成五种不同且高度保守的复制模式。核复制位点的组织可以通过电子显微镜和光学显微镜技术进行研究。本章描述了几种通过间接免疫荧光显微镜检测哺乳动物细胞核中复制灶的方法。