用荧光标记抗体观察组蛋白修饰的S期动态。

Observing S-phase dynamics of histone modifications with fluorescently labeled antibodies.

作者信息

Wu Rong, Terry Anna V, Gilbert David M

机构信息

Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, Syracuse, NY, USA.

出版信息

Methods Mol Biol. 2006;325:139-48. doi: 10.1385/1-59745-005-7:139.

DOI:10.1385/1-59745-005-7:139

PMID:16761725

Abstract

Histone modifications are central to epigenetic regulation and must be reestablished with each round of DNA replication. Here we describe methods to localize these modifications within mammalian nuclei and to relate them to specific spatiotemporal patterns of DNA replication.

摘要

组蛋白修饰是表观遗传调控的核心，并且必须在每一轮DNA复制时重新建立。在此，我们描述了在哺乳动物细胞核内定位这些修饰并将它们与DNA复制的特定时空模式相关联的方法。