Marins Mônica, Xavier Anna L R, Viana Nathan B, Fortes Fábio S A, Fróes Maira M, Menezes João R L
Laboratório de Neuroanatomia Celular, Programa de Anatomia, Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, RJ, Brazil.
Dev Neurobiol. 2009 Sep 15;69(11):715-30. doi: 10.1002/dneu.20737.
The massive migration of neuroblasts and young neurons through the anterior extension of the postnatal subventricular zone (SVZ), known as the rostral migratory stream (RMS) is still poorly understood on its molecular basis. In this work, we investigated the involvement of gap junctional communication (GJC) in the robust centrifugal migration from SVZ/RMS explants obtained from early postnatal (P4) rats. Cells were dye-coupled in homocellular and heterocellular pairings and expressed at least two connexins, Cx 43 and 45. Treatment with the uncoupler agent carbenoxolone (CBX, 10-100 microM) reversibly reduced outgrowth from SVZ explants, while its inactive analog, glycyrhizinic acid (GZA), had no effect. Consistent with a direct effect on cell migration, time-lapse video microscopy show that different pharmacological uncouplers cause an abrupt and reversible arrest of cell movement in explants. Our results indicate that GJC is positively involved in the migration of neuroblasts within the SVZ/RMS.
神经母细胞和年轻神经元通过出生后脑室下区（SVZ）的前延伸部分进行大规模迁移，即所谓的吻侧迁移流（RMS），其分子基础仍知之甚少。在这项研究中，我们调查了缝隙连接通讯（GJC）在出生后早期（P4）大鼠的SVZ/RMS外植体强大的离心迁移中的作用。细胞在同细胞和异细胞配对中进行染料偶联，并表达至少两种连接蛋白，即Cx 43和45。用解偶联剂羧苄青霉素（CBX，10 - 100微摩尔）处理可使SVZ外植体的生长可逆性减少，而其无活性类似物甘草酸（GZA）则无作用。与对细胞迁移的直接影响一致，延时视频显微镜显示不同的药理学解偶联剂会导致外植体中细胞运动突然且可逆地停止。我们的结果表明，GJC正向参与SVZ/RMS内神经母细胞的迁移。
