[黧豆质量标准研究]
[Study on quality standard of Mucuna pruriens var. utilis].
作者信息
Wu Shi-Hong, Jiang Wei-Zhe, Lv Li, Wu Ling-Ling, Lv Cong, Shi Xiao-Xia, Su Gui-Liang
机构信息
Guangxi Medical University, Nanning 530021, China.
出版信息
Zhong Yao Cai. 2009 Mar;32(3):333-5.
OBJECTIVE
To provide scientific basis for the utilization and development of Mucuna pruriens var. utilis by establishing its quality control standard.
METHODS
The bioactive constituents were analyzed by TLC and HPLC. Moisture, ash and the extracts of Mucuna pruriens var. utilis were all determined.
RESULTS
The TLC spots of levodopa had similar color with the control group at the same position. The results of HPLC quantitative analysis showed that the linear range of levodopa was 26.45 to approximately 132.25 microg/mL, r = 0.9992, and the average recovery rate was 103.8%, RSD = 1.85%.
CONCLUSIONS
This method is convenient, accurate, reliable with good reproducibility, so it can be used to establish quality standard for the medicinal material.
目的
通过建立黧豆的质量控制标准，为其资源利用与开发提供科学依据。
方法
采用薄层色谱法（TLC）和高效液相色谱法（HPLC）对生物活性成分进行分析。测定黧豆的水分、灰分及提取物。
结果
左旋多巴的TLC斑点在相同位置与对照组颜色相似。HPLC定量分析结果表明，左旋多巴的线性范围为26.45至约132.25μg/mL，r = 0.9992，平均回收率为103.8%，相对标准偏差（RSD）= 1.85%。
结论
该方法简便、准确、可靠，重现性好，可用于建立该药材的质量标准。
Zotero 插件