在磷酰基或酯氧位置标记有\(^{18}O\)的α-D-吡喃葡萄糖-1-磷酸酯的合成。

The syntheses of alpha-D-glucopyranose-1-phosphates labelled with 18O in the phosphoryl or ester oxygen positions.

作者信息

Kokesh F C, Kakuda Y

出版信息

Can J Biochem. 1977 May;55(5):548-54. doi: 10.1139/o77-078.

PMID:195685

Abstract

Phosphoryl-oxygen-labelled alpha-D-glucopyranose-1-phosphate (Glc1P) has been prepared by the hydrolysis in 18O-enriched water of alpha-D-glucopyranose-1,2-cyclic phosphate catalyzed by extracts of Saccharomyces fragilis. Ester-oxygen-labelled alpha-D-galactopyranose-1-phosphate has been prepared by galactokinase-(EC 2.7.1.6)-catalyzed phosphorylation of D-[1-18O]-galactose by ATP, and it has been isomerized to ester-oxygen-labelled Glc1P using galactose-1-phosphate uridyltransferase (EC2.7.7.12) and UDPgalactose 4-epimerase (EC 5.1.3.2). These labelled glucose phosphates are for use in oxygen-exchange experiments.

摘要

磷酸氧标记的α-D-吡喃葡萄糖-1-磷酸（Glc1P）是通过脆壁酵母提取物催化的α-D-吡喃葡萄糖-1,2-环磷酸在富含18O的水中水解制备的。酯氧标记的α-D-吡喃半乳糖-1-磷酸是通过半乳糖激酶-（EC 2.7.1.6）-催化ATP对D-[1-18O]-半乳糖的磷酸化制备的，并且使用半乳糖-1-磷酸尿苷酰转移酶（EC2.7.7.12）和UDP半乳糖4-差向异构酶（EC 5.1.3.2）将其异构化为酯氧标记的Glc1P。这些标记的葡萄糖磷酸用于氧交换实验。