Dragon N, Seidah N G, Lis M, Routhier R, Chrétien M
Can J Biochem. 1977 Jun;55(6):666-70. doi: 10.1139/o77-096.
The complete amino acid sequence of human beta-endorphin was obtained by automatic sequencing of a sulfonyl isothiocyanate derivative of this peptide, in combination with peptide mapping of a tryptic digest of the native molecule. It was found to be identical with the carboxy-terminal portion 61-91 of human beta-lipotropin (beta-LPH). The morphine-like activity of beta-endorphin is comparable both in the mouse vas deferens bioassay and in the opiate receptor binding assay. However, beta-LPH is not active up to concentrations of 10(-6) M.
通过对该肽的异硫氰酸磺酰衍生物进行自动测序，并结合天然分子胰蛋白酶消化产物的肽图谱分析，获得了人β-内啡肽的完整氨基酸序列。结果发现它与人β-促脂素（β-LPH）的羧基末端部分61-91相同。在小鼠输精管生物测定和阿片受体结合测定中，β-内啡肽的吗啡样活性相当。然而，在浓度高达10^(-6) M时，β-LPH没有活性。
