Temple University, Philadelphia, PA 19140, USA.
Drug Deliv. 1998;5(3):179-82. doi: 10.3109/10717549809052033.
All aqueous microencapsulation involving the use of the anionic polymer alginate as its amine salt with spermine has been recently studied for the encapsulation of live viruses and isolated proteins. It was of interest to study the influence of another anionic polymer, carrageenan, on the encapsulation of a single protein. Trypsin, used as a the model protein, was encapsulated in spermine carrageenan macrocapsules. It was found that trypsin retains its structure as evidenced by SDS-PAGE analysis and its functional integrity measured as specific enzymatic activity. Thus, carrageenan can serve as anionic polymer in all aqueous charged film encapsulation systems.
最近，人们研究了使用阴离子聚合物海藻酸钠的胺盐与亚精胺对活病毒和分离蛋白进行包封的所有水性微胶囊化方法。研究另一种阴离子聚合物卡拉胶对单一蛋白质包封的影响很有意义。胰蛋白酶被用作模型蛋白，被包封在亚精胺卡拉胶大胶囊中。研究发现，胰蛋白酶保留了其结构，这一点可以通过 SDS-PAGE 分析得到证明，并且可以通过测定其特异性酶活性来衡量其功能完整性。因此，卡拉胶可以作为所有水性带电膜包封系统中的阴离子聚合物。
