Silva A E F, Cavalcante L F, Rodrigues B A, Rodrigues J L
Laboratory of Embryology and Biotechnics of Reproduction, Faculty of Veterinary Medicine, UFRGS, Porto Alegre, RS, Brazil.
Reprod Domest Anim. 2010 Dec;45(6):1042-6. doi: 10.1111/j.1439-0531.2009.01487.x.
The objective of this study was to determine the influence of powdered coconut water (ACP-318(®)) diluted in high glucose (11.0 mM) TCM199 in the achievement of nuclear in vitro maturation (IVM) of canine oocytes. Cumulus oocyte complexes (COCs) (n = 632) were randomly allocated into three experimental groups named as group 1 (control group), group 2 (5% powdered coconut water) and group 3 (10% powdered coconut water). The percentage of meiotic resumption (MR) (GVBD to MII) was 39.1% (81/207), 50.2% (108/215) and 46.6% (98/210) for groups 1, 2 and 3 respectively (p < 0.05). There were no differences in MR rates among groups 2 and 3. The medium with ACP-318(®) slightly enhanced the nuclear maturation of canine oocytes when a comparison was established with rates of maturation exhibited by oocytes in the experimental group 1 without ACP-318(®) (p < 0.05). The results suggest that oocytes' nuclear morphology integrity and meiosis achievement were positively influenced when exposed to high glucose TCM199 supplemented with 5% powdered coconut water. Further investigation must be performed for a better understanding of powdered coconut water influence in cellular events during IVM of dog oocytes.
本研究的目的是确定在高糖（11.0 mM）的TCM199中稀释的椰子水粉末（ACP-318(®)）对犬卵母细胞体外核成熟（IVM）的影响。将卵丘卵母细胞复合体（COCs）（n = 632）随机分为三个实验组，分别命名为第1组（对照组）、第2组（5%椰子水粉末）和第3组（10%椰子水粉末）。第1、2和3组的减数分裂恢复（MR）（从GVBD到MII）百分比分别为39.1%（81/207）、50.2%（108/215）和46.6%（98/210）（p < 0.05）。第2组和第3组之间的MR率没有差异。与不含ACP-318(®)的第1实验组卵母细胞的成熟率相比，含有ACP-318(®)的培养基略微提高了犬卵母细胞的核成熟率（p < 0.05）。结果表明，当暴露于添加5%椰子水粉末的高糖TCM199时，卵母细胞的核形态完整性和减数分裂完成受到积极影响。必须进行进一步研究，以更好地了解椰子水粉末对犬卵母细胞IVM期间细胞事件的影响。
