Orthodontic Unit, School of Dentistry, The University of Adelaide, Frome Road, Adelaide, South Australia 5005, Australia.
Arch Oral Biol. 2009 Sep;54(9):871-8. doi: 10.1016/j.archoralbio.2009.06.003. Epub 2009 Jun 30.
Nerve growth factor (NGF) and its receptors, p75 and tyrosine receptor kinase A (Trk A), have been shown to increase following trauma. The aims of this study were to examine changes in the detection of NGF and its receptors during orthodontic tooth movement in the rat, and the effects of anti-NGF on these changes.
Orthodontic separators were placed between the right maxillary first and second molars of Sprague-Dawley rats which were equally divided into two groups. Animals from the second group were injected with anti-NGF. The left sides served as controls, and animals were sacrificed at 0, 3, 7 and 14 days.
Results of immunohistochemical localisation for p75, Trk A, calcitonin gene-related peptide (CGRP) and NGF showed staining intensity increased at day 3, with a peak at day 7 and decreasing intensity at day 14. Anti-NGF injected animals showed reduced staining at all observation periods.
Data suggest that orthodontic injury induces NGF production, leading to sprouting and invasion by CGRP-positive nerve fibers and that injection of anti-NGF reduces NGF tissue levels and prevents innervation by CGRP-positive fibers.
神经生长因子（NGF）及其受体 p75 和酪氨酸受体激酶 A（TrkA）在创伤后会增加。本研究旨在检测大鼠正畸牙齿移动过程中 NGF 及其受体的检测变化，以及抗 NGF 对这些变化的影响。
在 Sprague-Dawley 大鼠的右侧上颌第一和第二磨牙之间放置正畸分离剂，将动物等分为两组。第二组动物注射抗 NGF。左侧作为对照，动物在 0、3、7 和 14 天处死。
p75、TrkA、降钙素基因相关肽（CGRP）和 NGF 的免疫组织化学定位结果显示，染色强度在第 3 天增加，第 7 天达到峰值，第 14 天强度降低。在所有观察期，注射抗 NGF 的动物显示染色减少。
数据表明，正畸损伤诱导 NGF 产生，导致 CGRP 阳性神经纤维的发芽和浸润，而注射抗 NGF 可降低 NGF 组织水平并防止 CGRP 阳性纤维的神经支配。
