Plk1 的过表达促进人食管鳞癌细胞的恶性进展。
Overexpression of Plk1 promotes malignant progress in human esophageal squamous cell carcinoma.
机构信息
Basic Medicine Department, Weifang Medical University, 261053 Weifang, Shandong, People's Republic of China.
出版信息
J Cancer Res Clin Oncol. 2010 Jan;136(1):9-16. doi: 10.1007/s00432-009-0630-4.
PURPOSE
Plk1, belonging to a family of serine/threonine kinases, is involved in spindle formation and chromosome segregation during mitosis and therefore, in the regulation of cell cycle. Plk1 was found to be overexpressed in various human tumors. In the present work, we investigated the expression of human esophageal squamous cell carcinoma (ESCC) to determine whether Plk1 has a role in malignant progress.
METHODS
Immunohistochemistry and Western blotting were performed to define the expressions of Plk1 in ESCC tissues and normal adjacent tissues. Transfection of cells with small interference RNA, growth suppression assay, and Transwell assay were used to determine the potential role of Plk1 in ESCC malignant progress.
RESULTS
Plk1 was overexpressed in 69.6% of the ESCC tissues. In addition, the extent of Plk1 expression was closely correlated with differentiation grades and invasiveness in ESCC. We also found that the downregulation of endogenous Plk1 in human ESSC cell lines Eca-109 and EC9706 significantly decreased cells proliferation and migrating ability.
CONCLUSIONS
Our results show that Plk1 expression is elevated in ESCC tissues and is associated with differentiation grades and invasiveness in ESCC, indicating that overexpression of Plk1 may play an important role in carcinogenesis and malignant progress of ESCC.
目的
Plk1 属于丝氨酸/苏氨酸激酶家族，参与有丝分裂过程中的纺锤体形成和染色体分离，从而调节细胞周期。Plk1 在各种人类肿瘤中过表达。本研究检测了人食管鳞癌（ESCC）组织中 Plk1 的表达，以确定 Plk1 是否在恶性进展中发挥作用。
方法
采用免疫组化和 Western blot 检测 Plk1 在 ESCC 组织和正常相邻组织中的表达。用小干扰 RNA 转染细胞、生长抑制实验和 Transwell 实验，以确定 Plk1 在 ESCC 恶性进展中的潜在作用。
结果
69.6%的 ESCC 组织中 Plk1 过表达。此外，Plk1 的表达程度与 ESCC 的分化程度和侵袭性密切相关。我们还发现，人 ESCC 细胞系 Eca-109 和 EC9706 中内源性 Plk1 的下调显著降低了细胞的增殖和迁移能力。
结论
我们的结果表明，Plk1 在 ESCC 组织中表达上调，并与 ESCC 的分化程度和侵袭性相关，表明 Plk1 的过表达可能在 ESCC 的发生和恶性进展中发挥重要作用。
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