聚己内酯涂覆多孔磷酸三钙支架用于组织工程中蛋白质的控制释放。
Polycaprolactone coated porous tricalcium phosphate scaffolds for controlled release of protein for tissue engineering.
机构信息
W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164.
出版信息
J Biomed Mater Res B Appl Biomater. 2009 Nov;91(2):831-838. doi: 10.1002/jbm.b.31464.
Polycaprolactone (PCL) was coated on porous tricalcium phosphate (TCP) scaffolds to achieve controlled protein delivery. Porous TCP scaffolds were fabricated using reticulated polyurethane foam as sacrificial scaffold with a porosity of 70-90 vol %. PCL was coated on sintered porous TCP scaffolds by dipping-drying process. The compressive strength of TCP scaffolds increased significantly after PCL coating. The highest strength of 2.41 MPa at a porosity of 70% was obtained for the TCP scaffold coated with 5% PCL solution. Model protein bovine serum albumin (BSA) was encapsulated efficiently within the PCL coating. The amount of BSA encapsulation was controlled by varying proteins' composition in the PCL coating. The FTIR analysis confirmed that BSA retained its structural conformation and did not show significant denaturization during PCL coating. The release kinetics in phosphate buffer solution indicated that the protein release was controlled and sustained, and primarily dependant on protein concentration encapsulated in the PCL coating.
聚己内酯（PCL）被涂覆在多孔磷酸三钙（TCP）支架上，以实现蛋白质的控制释放。多孔 TCP 支架采用泡沫聚氨酯作为牺牲支架来制备，其孔隙率为 70-90vol%。PCL 通过浸渍-干燥工艺涂覆在烧结多孔 TCP 支架上。PCL 涂层显著提高了 TCP 支架的抗压强度。在 70%孔隙率下，PCL 溶液浓度为 5%的 TCP 支架的抗压强度最高，达到 2.41MPa。模型蛋白牛血清白蛋白（BSA）被有效地包裹在 PCL 涂层内。通过改变 PCL 涂层中蛋白质的组成，可以控制 BSA 的包封量。傅里叶变换红外分析（FTIR）证实 BSA 在 PCL 涂层过程中保持其结构构象，没有明显的变性。在磷酸盐缓冲溶液中的释放动力学表明，蛋白质的释放是可控和持续的，主要依赖于 PCL 涂层中包封的蛋白质浓度。
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