Klotz U
Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Stuttgart, F.R.G.
Ther Drug Monit. 1991 Sep;13(5):461-3. doi: 10.1097/00007691-199109000-00012.
Whole blood steady-state trough concentrations of cyclosporine were measured by radioimmunoassay (RIA) and TDx assays employing monoclonal antibodies in 82 samples from 39 renal transplant patients. Intra- and interassay coefficients of variation for the new TDx technique averaged 3.2 and 5.4%, respectively. In the concentration range of 40 to 717 ng/ml, there was a linear correlation (r = 0.884, p less than 0.001) between both assays [slope of 0.863; y intercept (RIA values) = 15 ng/ml], indicating that the methods can be used interchangeably. Since the automated TDx assay is faster and causes less waste problems, this reliable technique offers some definite advantages for routine clinical use.
采用放射免疫分析法（RIA）和运用单克隆抗体的TDx分析法，对39例肾移植患者的82份样本进行了环孢素全血稳态谷浓度测定。新的TDx技术的批内和批间变异系数平均分别为3.2%和5.4%。在40至717 ng/ml的浓度范围内，两种检测方法之间存在线性相关性（r = 0.884，p < 0.001）[斜率为0.863；截距（RIA值）= 15 ng/ml]，这表明两种方法可交替使用。由于自动化的TDx检测速度更快且产生的废物问题更少，这种可靠的技术在常规临床应用中具有一些明显优势。
