Fourrier M C S, Arnold M F F, Collet B, Munro E S
Marine Scotland, Marine Laboratory, Aberdeen, UK.
Fish Shellfish Immunol. 2009 Sep;27(3):535-8. doi: 10.1016/j.fsi.2009.06.017. Epub 2009 Jun 30.
Over sub-culturing a cell line generates a selective pressure which can result in key cellular functions being altered such as gene and protein expression. The present study set out to determine whether serial sub-culturing affects the antiviral state of the Salmon Head Kidney (SHK-1) cell line. Cells were cultured under constant conditions and real-time PCR was performed to measure the level of interferon (IFN) and Mx gene expression over different passage numbers. A significant increase in the basal level of IFN and Mx gene expression was recorded at passage number 58 (3 and 14-fold increase versus passage number 53), suggesting a sub-culturing effect on the type I IFN response in SHK-1 cells. Passage dependent variations in morphology and cell sub-populations have been previously observed in SHK-1 cells. Such variations in cell sub-types were suspected to be responsible for the fluctuations in IFN and Mx gene expression recorded in this study.
对细胞系进行多次传代培养会产生选择性压力，这可能导致关键细胞功能发生改变，如基因和蛋白质表达。本研究旨在确定连续传代培养是否会影响鲑鱼头肾（SHK-1）细胞系的抗病毒状态。细胞在恒定条件下培养，并通过实时PCR检测不同传代次数下干扰素（IFN）和Mx基因的表达水平。在第58代时，IFN和Mx基因的基础表达水平显著增加（与第53代相比分别增加了3倍和14倍），表明传代培养对SHK-1细胞中的I型IFN反应有影响。先前在SHK-1细胞中观察到形态和细胞亚群的传代依赖性变化。怀疑这种细胞亚型的变化是本研究中记录的IFN和Mx基因表达波动的原因。
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