Raghunathan Kannan, Vago Frank S, Ball Terry, Yakubova Nafissa, Grindem David, Wedemeyer William J, Arvidson Dennis N
Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jul 1;65(Pt 7):702-4. doi: 10.1107/S1744309109020454. Epub 2009 Jun 27.
EpsH is a minor pseudopilin protein of the Vibrio cholerae type II secretion system. A truncated form of EpsH with a C-terminal noncleavable His tag was constructed and expressed in Escherichia coli, purified and crystallized by sitting-drop vapor diffusion. A complete data set was collected to 1.71 A resolution. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 53.39, b = 71.11, c = 84.64 A. There were two protein molecules in the asymmetric unit, which gave a Matthews coefficient V(M) of 2.1 A(3) Da(-1), corresponding to 41.5% solvent content.
EpsH是霍乱弧菌II型分泌系统的一种次要假菌毛蛋白。构建了一种带有C端不可切割His标签的EpsH截短形式，并在大肠杆菌中表达，通过坐滴气相扩散法进行纯化和结晶。收集了分辨率为1.71 Å的完整数据集。晶体属于空间群P2(1)2(1)2(1)，晶胞参数a = 53.39、b = 71.11、c = 84.64 Å。不对称单元中有两个蛋白质分子，其马修斯系数V(M)为2.1 ų Da⁻¹，对应溶剂含量为41.5%。
