通过接枝pH响应性聚合物制备整体柱及其表征

Preparation and characterization of monolithic column by grafting pH-responsive polymer.

作者信息

Wei Xiaoyi, Qi Li, Yang Gengliang, Wang Fuyi

机构信息

Beijing National Laboratory for Molecular Sciences, Laboratory of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, No. 2 North First Street, Zhongguancun, Beijing 100190, China.

出版信息

Talanta. 2009 Aug 15;79(3):739-45. doi: 10.1016/j.talanta.2009.04.062. Epub 2009 May 7.

DOI:10.1016/j.talanta.2009.04.062

PMID:19576439

Abstract

A novel modified monolithic column with pH-responsive polymer chains was prepared by grafting methacrylic acid onto the poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith. The grafting polymerization was achieved in an in situ manner which was performed by pumping methacrylic acid directly through an acidic hydrolysis monolithic column using potassium peroxydisulfate initiated free-radical polymerization. The grafted monolithic column was demonstrated to be the pH-responsive to the pore structure and the chromatographic characterization. The permeability of the column and the retention factors of five benzene homologues decreased due to the conformational changes of the polymer chains when the pH of mobile phase increased from 4.5 to 7.5. Furthermore, the modified monolithic column was used as the pH-responsive stationary phase and exhibited an excellent separation of four basic proteins.

摘要

通过将甲基丙烯酸接枝到聚（甲基丙烯酸缩水甘油酯 - 二甲基丙烯酸乙二醇酯）整体柱上，制备了一种具有pH响应聚合物链的新型改性整体柱。接枝聚合以原位方式实现，即通过使用过硫酸钾引发的自由基聚合将甲基丙烯酸直接泵入酸性水解整体柱中来进行。结果表明，接枝整体柱对孔结构和色谱表征具有pH响应性。当流动相的pH值从4.5增加到7.5时，由于聚合物链的构象变化，柱的渗透率和五种苯同系物的保留因子降低。此外，改性整体柱用作pH响应固定相，对四种碱性蛋白质表现出优异的分离效果。