Viklund C, Svec F, Fréchet J M, Irgum K
Department of Chemistry, University of California, Berkeley 94720-1460, USA.
Biotechnol Prog. 1997 Sep-Oct;13(5):597-600. doi: 10.1021/bp9700667.
Porous poly(glycidyl methacrylate-co-ethylene dimethacrylate) monoliths with different porous properties grafted with poly(2-acrylamido-2-methyl-1-propanesulfonic acid) chains using cerium(IV) initiated free-radical polymerization have been prepared and used for the separation of proteins in ion-exchange HPLC mode. Because of the presence of the large pores that are typical of monolithic separation media which allow easy flow of all of the mobile phase, the efficiency of the columns does not deteriorate even at high flow velocities as a result of the specific morphology of the monoliths. Optimization of the chromatographic conditions such as the shape of the mobile phase gradient and the flow rate allows for very fast separation of three proteins in less than 1.5 min.
通过铈（IV）引发自由基聚合制备了具有不同多孔性质的聚（甲基丙烯酸缩水甘油酯-共-二甲基丙烯酸乙烯酯）整体柱，并用聚（2-丙烯酰胺-2-甲基-1-丙烷磺酸）链进行接枝，用于离子交换高效液相色谱模式下蛋白质的分离。由于整体分离介质中典型的大孔的存在，使得所有流动相都能轻松流动，由于整体柱的特殊形态，即使在高流速下，柱效也不会降低。对色谱条件（如流动相梯度形状和流速）进行优化后，可在不到1.5分钟的时间内非常快速地分离三种蛋白质。
