Stepnik Maciej, Stetkiewicz Jan, Krajnow Aleksander, Domeradzka Katarzyna, Gradecka-Meesters Dobrosława, Arkusz Joanna, Stańczyk Małgorzata, Palus Jadwiga, Dziubałtowska Elzbieta, Sobala Wojciech, Gromadzińska Jolanta, Wasowicz Wojciech, Rydzyński Konrad
Nofer Institute of Occupational Medicine, 8 Sw. Teresy Street, 91-348 Łódź, Poland.
Ecotoxicol Environ Saf. 2009 Nov;72(8):2143-52. doi: 10.1016/j.ecoenv.2009.06.005. Epub 2009 Jul 3.
In this study, carcinogenic effects of arsenate in female C57BL/6J/Han mice exposed in drinking water to 50, 200 or 500microgAs/L for 24 months were investigated. All animals were fed low-selenium diet, however half of them were supplemented with sodium selenite in drinking water (200microgSe/L) to ensure the normal dietary level of selenium. Glutathione peroxidase activity in erythrocytes and plasma as well as selenium concentration in plasma after 3, 6, 12 and 18 months in satellite groups showed considerable decrease in animals from non-selenium supplemented groups in comparison to supplemented groups. A clear arsenic concentration-dependent increase in the number of malignant lymphoma associated with increase in the risk of death was observed (hazard ratio=0.91, 1.46, and 2.24, for 50, 200 and 500microgAs/L, respectively). No significant influence of selenium dietary status on arsenic carcinogenicity was shown. A significant association between selenium supplementation status and increased risk of death of the animals from causes other than malignant tumors was found (HR=1.79, p=0.04).
在本研究中，调查了雌性C57BL/6J/Han小鼠饮用含50、200或500μg As/L砷酸盐的水24个月后的致癌作用。所有动物均喂食低硒饮食，但其中一半动物在饮用水中补充亚硒酸钠（200μg Se/L）以确保正常的膳食硒水平。卫星组在3、6、12和18个月后，未补充硒的组动物红细胞和血浆中的谷胱甘肽过氧化物酶活性以及血浆中的硒浓度与补充硒的组相比显著降低。观察到与死亡风险增加相关的恶性淋巴瘤数量呈明显的砷浓度依赖性增加（50、200和500μg As/L的风险比分别为0.91、1.46和2.24）。未显示硒膳食状态对砷致癌性有显著影响。发现补充硒状态与动物因恶性肿瘤以外的原因导致的死亡风险增加之间存在显著关联（风险比=1.79，p=0.04）。
