Jeong Injae, Kim Beom-Soo, Lee Hyejung, Lee Kang-Min, Shim Insop, Kang Sung-Keel, Yin Chang-Shick, Hahm Dae-Hyun
Acupuncture and Meridian Science Research Center, Kyung Hee University, Seoul, Republic of Korea.
Int J Pharm. 2009 Oct 1;380(1-2):62-6. doi: 10.1016/j.ijpharm.2009.06.034. Epub 2009 Jul 3.
To enhance the medicinal activity of bee venom (BV) acupuncture, bee venom was loaded into biodegradable poly(D,L-lactide-co-glycolide) nanoparticles (BV-PLGA-NPs) by a water-in-oil-in-water-emulsion/solvent-evaporation technique. Rat formalin tests were performed after subcutaneous injection of BV-PLGA-NPs to the Zusanli acupuncture point (ST36) at 0.5, 1, 2, 6, 12, 24, and 48 h before plantar injection of 2% formalin. BV-PLGA-NPs treatment showed comparable analgesic activity to typical BV acupuncture during the late phase, compared with saline-treated controls, and the analgesic effect lasted for 12h. PLGA-encapsulation was also effective in alleviating the edema induced by allergens in bee venom. These results indicate that PLGA-encapsulation provided a more prolonged effect of BV acupuncture treatment, while maintaining a comparable therapeutic effect.
为增强蜂毒针刺疗法的药用活性，采用水包油包水乳液/溶剂蒸发技术将蜂毒载入可生物降解的聚（D，L-丙交酯-共-乙交酯）纳米颗粒（BV-PLGA-NPs）中。在足底注射2%福尔马林前0.5、1、2、6、12、24和48小时，将BV-PLGA-NPs皮下注射到足三里穴位（ST36）后进行大鼠福尔马林试验。与生理盐水处理的对照组相比，BV-PLGA-NPs治疗在后期显示出与典型蜂毒针刺相当的镇痛活性，且镇痛效果持续12小时。PLGA包封对减轻蜂毒中过敏原引起的水肿也有效。这些结果表明，PLGA包封在维持相当治疗效果的同时，延长了蜂毒针刺治疗的效果。
