Rabaste F, Dauphin G, Jeminet G, Guyot J, Delort A M
Université Blaise Pascal Clermont-Ferrand, Laboratoire de Chimie Organique Biologique-U.R.A., 485 du C.N.R.S., Aubière, France.
Biochem Biophys Res Commun. 1991 Nov 27;181(1):74-9. doi: 10.1016/s0006-291x(05)81383-x.
Na+ movements in S. faecalis were studied by 23Na NMR. They proved to be dependent on phosphate concentration in the buffer during the de-energization step. K+ and H+ were also studied respectively by potentiometry and 31P NMR and were shown not to be implicated. For de-energized cells the internal phosphate concentration, on the contrary, was directly linked to the external phosphate contained in the buffer. The experiments showed a Na+/Pi dependence in this prokaryote so far known only in eukaryotes.
利用23Na核磁共振技术研究了粪肠球菌中的钠离子运动。结果表明，在去能步骤中，它们依赖于缓冲液中磷酸盐的浓度。还分别通过电位滴定法和31P核磁共振技术研究了钾离子和氢离子，结果表明它们没有牵涉其中。相比之下，对于去能细胞，内部磷酸盐浓度与缓冲液中所含的外部磷酸盐直接相关。实验表明，这种原核生物中存在钠离子/无机磷酸盐依赖性，而迄今为止仅在真核生物中才知晓这种依赖性。
