Kitawaki Ryoko, Nishimura Yuko, Takagi Naohiro, Iwasaki Mitsuhiro, Tsuzuki Kimiko, Fukuda Mitsuru
Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women's University, Nishinomiya, Hyogo, Japan.
Biosci Biotechnol Biochem. 2009 Jul;73(7):1484-8. doi: 10.1271/bbb.80753. Epub 2009 Jul 7.
We examined the effects of lactic acid fermented soymilk, in which part of the soymilk was replaced with okara (soy yogurt), on plasma and hepatic lipid profiles in rats fed a cholesterol-free diet. Additionally, we investigated the effects of soy yogurt on hepatic gene expression in rats using DNA microarray analysis. Male Sprague-Dawley rats aged 5 weeks (n=5/group) were fed a control diet (AIN-93) or a test diet in which 20% of the diet was replaced by soy yogurt for 7 weeks. Soy yogurt consumption did not affect body weight or adipose tissue weight as compared with control diet. In the soy yogurt group, the liver weight and hepatic triglyceride content were significantly lower than the control group, and the level of plasma cholesterol was also lower. Furthermore, DNA microarray analysis indicated that soy yogurt ingestion down-regulated the expression of the SREBP-1 gene and enzymes related to lipogenesis in the rat liver, while expression of beta-oxidation-related genes was up-regulated. These results suggest that soy yogurt is beneficial in preventing hepatic lipid accumulation in rats.
我们研究了用豆渣（大豆酸奶）替代部分豆浆制成的乳酸发酵豆浆对食用无胆固醇饮食的大鼠血浆和肝脏脂质谱的影响。此外，我们使用DNA微阵列分析研究了大豆酸奶对大鼠肝脏基因表达的影响。将5周龄的雄性Sprague-Dawley大鼠（每组n = 5）喂食对照饮食（AIN-93）或用大豆酸奶替代20%饮食的试验饮食，持续7周。与对照饮食相比，食用大豆酸奶不影响体重或脂肪组织重量。在大豆酸奶组中，肝脏重量和肝脏甘油三酯含量显著低于对照组，血浆胆固醇水平也较低。此外，DNA微阵列分析表明，摄入大豆酸奶可下调大鼠肝脏中SREBP-1基因和与脂肪生成相关的酶的表达，而与β-氧化相关的基因表达上调。这些结果表明，大豆酸奶有利于预防大鼠肝脏脂质积累。
