Livigni Alessandra, Villegas Santiago Nahuel, Oikonomopoulou Ifigenia, Rahman Afifah, Morrison Gillian M, Brickman Joshua M
MRC Centre for Regenerative Medicine, Institute for Stem Cell Research, University of Edinburgh, Edinburgh, United Kingdom.
Curr Protoc Stem Cell Biol. 2009 Jul;Chapter 1:Unit 1G.3. doi: 10.1002/9780470151808.sc01g03s10.
Anterior definitive endoderm (ADE) is both an important embryonic signaling center and a unique multipotent precursor of liver, pancreas, and other visceral organs. Here we describe a method for the differentiation of mouse embryonic stem (ES) cells to endoderm with pronounced anterior character. ADE-containing cultures can be produced in vitro by suspension (aggregation or embryoid body) culture and in a serum-free adherent monolayer culture. Purified ES cell-derived ADE cells appear committed to endodermal fates and can undergo further differentiation in vitro towards liver and pancreas with enhanced efficiency.
前侧定形内胚层（ADE）既是一个重要的胚胎信号中心，也是肝脏、胰腺和其他内脏器官独特的多能前体细胞。在此，我们描述了一种将小鼠胚胎干细胞（ES细胞）分化为具有明显前侧特征的内胚层细胞的方法。含ADE的培养物可通过悬浮（聚集或胚状体）培养以及无血清贴壁单层培养在体外产生。纯化的ES细胞来源的ADE细胞似乎已确定向内胚层命运分化，并且能够在体外以更高的效率进一步分化为肝脏和胰腺细胞。
