Leibowitz D, Young K, Cox G
Department of Medicine, Indiana University School of Medicine, Indianapolis.
Genes Chromosomes Cancer. 1991 Jul;3(4):308-12. doi: 10.1002/gcc.2870030410.
The Philadelphia1 (Ph1) chromosome results from a reciprocal translocation between chromosome 9 and chromosome 22, which fuses a portion of the ABL oncogene to the BCR gene, forming the BCR/ABL fusion gene. This produces a fusion protein with a greatly increased protein tyrosine kinase activity in comparison to that of the normal ABL protein. The BCR/ABL gene is transcribed from the promoter of the normal BCR gene, but little is known about the regulation of its expression. In this study, we asked whether there are sequence-specific DNA-binding proteins (DBP) that bind to the breakpoint cluster region (bcr, or Mbcr) within the BCR gene. Sequence-specific DBP located within the Mbcr could have a transcription-regulating effect, and they could participate in the recombination that generates BCR/ABL. Our data show that there are sequence-specific DBP that bind within the Mbcr.
费城1（Ph1）染色体源于9号染色体和22号染色体之间的相互易位，该易位将ABL癌基因的一部分与BCR基因融合，形成BCR/ABL融合基因。与正常ABL蛋白相比，这产生了一种蛋白酪氨酸激酶活性大大增加的融合蛋白。BCR/ABL基因从正常BCR基因的启动子转录，但对其表达调控知之甚少。在本研究中，我们探究是否存在与BCR基因内的断裂点簇区域（bcr，或Mbcr）结合的序列特异性DNA结合蛋白（DBP）。位于Mbcr内的序列特异性DBP可能具有转录调节作用，并且它们可能参与产生BCR/ABL的重组过程。我们的数据表明，存在与Mbcr内结合的序列特异性DBP。
