Kawamoto K, Fujiwara H, Numa Y, Matsumura H
Department of Neurosurgery, Kansai Medical University.
Hum Cell. 1991 Jun;4(2):157-64.
For the purpose of FCM, we have developed a new method of sterile sorting of NK cells by negative selection using FITC-labelled monoclonal antibodies. In this method, over 95% lymphocytes with large granular lymphocytes (NK cells) were obtained by exclusion of Leu-1 and HLA-DR positive cells from human peripheral blood. From the results of cytotoxicity test of NK cells against 4 types of glioma cell lines and 6 kinds of cultured cells from surgical materials, the antineoplastic effect was recognized in 4 out of 6 surgical materials. Administration of NK cells through an Ommaya tube in 2 brain tumor patients caused no side effects. This technique is clinically applicable because 1) NK cells are easy to obtain, 2) NK cells show antineoplastic effect on brain tumors, and 3) the sensitivity can be evaluated in individual patients.
为了进行流式细胞术（FCM）分析，我们开发了一种新的通过使用异硫氰酸荧光素（FITC）标记的单克隆抗体进行阴性选择来无菌分选自然杀伤（NK）细胞的方法。在该方法中，通过从人外周血中排除亮氨酸-1（Leu-1）和人类白细胞抗原-DR（HLA-DR）阳性细胞，获得了超过95%的具有大颗粒淋巴细胞（即NK细胞）的淋巴细胞。根据NK细胞对4种胶质瘤细胞系和6种手术材料培养细胞的细胞毒性测试结果，在6种手术材料中有4种观察到了抗肿瘤作用。对2例脑肿瘤患者通过Ommaya管注射NK细胞未引起副作用。该技术在临床上具有应用价值，原因如下：1）NK细胞易于获取；2）NK细胞对脑肿瘤显示出抗肿瘤作用；3）可以在个体患者中评估敏感性。
