Wu Chiping, Wais Marta, Zahid Tariq, Wan Qi, Zhang Liang
Toronto Western Research Institute, Toronto, Ontario, Canada.
Behav Res Methods. 2009 Aug;41(3):736-41. doi: 10.3758/BRM.41.3.736.
We recently developed a glue-based method for the implantation of intracranial electrodes in mice. Our approach is to secure a preconstructed electrode array using a cyanoacrylate-based glue (similar to Krazy Glue). This method is applicable to both young and aging mice and is suitable for long-term electroencephalographic recordings. In the present experiment, we explored whether the glue-based method is capable of securing individual electrodes in addition to securing the electrode array. C57 black mice aged 25-35 days or 13-19 months were operated on under isoflurane anesthesia. Monopolar or bipolar electrodes were inserted independently in the ipsilateral hippocampal CA3 and entorhinal cortical areas, and they were fixed onto the skull using the glue together with dental acrylic, but without anchoring screws. We found that the implanted electrodes were stable and allowed repeat intracranial recordings and electrical stimulation in freely moving mice.
我们最近开发了一种基于胶水的方法，用于在小鼠体内植入颅内电极。我们的方法是使用一种基于氰基丙烯酸酯的胶水（类似于强力胶）来固定预先构建好的电极阵列。这种方法适用于年轻和老龄小鼠，并且适合进行长期脑电图记录。在本实验中，我们探究了基于胶水的方法除了固定电极阵列外，是否还能够固定单个电极。对25至35天龄或13至19月龄的C57黑小鼠在异氟烷麻醉下进行手术。将单极或双极电极独立插入同侧海马CA3区和内嗅皮质区，然后使用胶水和牙科丙烯酸树脂将它们固定在颅骨上，但不使用固定螺钉。我们发现植入的电极很稳定，并且允许在自由活动的小鼠中进行重复的颅内记录和电刺激。
