Muro Enrique M, McCann Jennifer A, Rudnicki Michael A, Andrade-Navarro Miguel A
Max-Delbrück Center for Molecular Medicine, Berlin, Germany.
Methods Mol Biol. 2009;567:145-54. doi: 10.1007/978-1-60327-414-2_10.
The simultaneous genotyping of thousands of single nucleotide polymorphisms (SNPs) in a genome using SNP-Arrays is a very important tool that is revolutionizing genetics and molecular biology. We expanded the utility of this technique by using it following chromatin immunoprecipitation (ChIP) to assess the multiple genomic locations protected by a protein complex recognized by an antibody. The power of this technique is illustrated through an analysis of the changes in histone H4 acetylation, a marker of open chromatin and transcriptionally active genomic regions, which occur during differentiation of human myoblasts into myotubes. The findings have been validated by the observation of a significant correlation between the detected histone modifications and the expression of the nearby genes, as measured by DNA expression microarrays. This chapter focuses on the computational analysis of the data.
使用单核苷酸多态性（SNP）芯片对基因组中的数千个单核苷酸多态性进行同步基因分型是一项非常重要的工具，正在革新遗传学和分子生物学。我们通过在染色质免疫沉淀（ChIP）后使用该技术来评估由抗体识别的蛋白质复合物所保护的多个基因组位置，从而扩展了这项技术的用途。通过分析组蛋白H4乙酰化的变化（开放染色质和转录活性基因组区域的标志物）来说明该技术的强大功能，这种变化发生在人类成肌细胞分化为肌管的过程中。通过观察检测到的组蛋白修饰与附近基因的表达之间的显著相关性（通过DNA表达微阵列测量），这些发现得到了验证。本章重点关注数据的计算分析。
