de Moraes Natália Valadares, Moretti Raquel Alves Corrêa, Furr Edward B, McCurdy Christopher R, Lanchote Vera Lucia
Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP 14040-903, Brazil.
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 Aug 15;877(24):2593-7. doi: 10.1016/j.jchromb.2009.06.023. Epub 2009 Jun 21.
This study used for the first time LC-MS/MS for the analysis of mitragynine (MIT), a mu-opioid agonist with antinociceptive and antitussive properties, in rat plasma. Mitragynine and the internal standard (amitriptyline) were extracted from plasma with hexane-isoamyl alcohol and resolved on a Lichrospher RP-SelectB column (9.80 and 12.90 min, respectively). The quantification limit was 0.2 ng/mL within a linear range of 0.2-1000 ng/mL. The method was applied to quantify mitragynine in plasma samples of rats (n=8 per sampling time) treated with a single oral dose of 20 mg/kg. The following pharmacokinetic parameters were obtained (mean): maximum plasma concentration: 424 ng/mL; time to reach maximum plasma concentration: 1.26 h; elimination half-life: 3.85 h, apparent total clearance: 6.35 L/h/kg, and apparent volume of distribution: 37.90 L/kg.
本研究首次使用液相色谱-串联质谱法（LC-MS/MS）分析大鼠血浆中的米托蒽醌（MIT），这是一种具有镇痛和镇咳特性的μ-阿片受体激动剂。米托蒽醌和内标（阿米替林）用己烷-异戊醇从血浆中提取，并在Lichrospher RP-SelectB柱上分离（分别为9.80和12.90分钟）。在0.2-1000 ng/mL的线性范围内，定量限为0.2 ng/mL。该方法用于定量单次口服20 mg/kg的大鼠（每个采样时间n = 8）血浆样品中的米托蒽醌。获得以下药代动力学参数（平均值）：最大血浆浓度：424 ng/mL；达到最大血浆浓度的时间：1.26小时；消除半衰期：3.85小时，表观总清除率：6.35 L/h/kg，表观分布容积：37.90 L/kg。
