香苓骨宝对去卵巢大鼠股骨骨折愈合的影响

Mu Shulin, Zhang Liu, Li Zhen, Li Qiang, Cheng Tan, Tian Faming, Liu Yongqiang

Department of Orthopaedic Surgery, the Affiliated Hospital of North China Coal Medical College, Tangshan Hebei, 063000, PR China.

Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2009 Jun;23(6):690-3.

OBJECTIVE

To evaluate the effects of XiangLingGuBao (XLGB) on femoral fracture healing in ovariectomized (OVX) rats.

METHODS

Forty 12-week-old female SD rats weighing (258 +/- 14) g were divided randomly into 4 groups (n = 10 per group): group A, sham operation by opening the abdominal cavity; group B, bilateral ovariectomy; group C, bilateral ovariectomy, transverse midshaft fracture of the right femur with intramedullary nail fixation, and normal saline by gavage; group D, bilateral ovariectomy, transverse midshaft fracture of the right femur with intramedullary nail fixation, and 250 mg/(kg x d) XLGB by gavage. The weight of rabbits in groups A and B was measured 0, 1, 2, 3, 4 and 5 weeks after operation. The right femur of each rat was obtained 5 weeks after operation. Total femur bone mineral density (tBMD), distal femur bone mineral density (dBMD) and middle femur bone mineral density (mBMD) were measured by double energy X-ray absorptiometry CR filming, HE staining and immunohistochemistry staining of groups C and D were performed.

RESULTS

The weight of rats in group B was obviously higher than that of group A at 3, 4 and 5 weeks after operation (P < 0.05), indicating the animal model of postmenopausal osteoporosis was established successfully. CR films showed more callus and obscure fracture line in group D, while less callus and distinct fracture line in group C. The tBMD and the dBMD of group B were far less than that of group A, the mBMD of group D was significantly higher than that of group C (P < 0.05), the tBMD and the dBMD of group D were higher than that of group C, but no significant difference was evident between two groups (P > 0.05). Histology observation showed, when compared with group C, most fracture ends in group D reached bone union, and the introduction of capillaries was evident in the marrow cavity. Immunohistochemistry staining demonstrated that the BMP-2 integrated absorbance (IA) value in groups C and D was 2.236 6 +/- 0.181 8 and 3.727 3 +/- 0.874 2, respectively, the VEGF IA value in groups C and D was 2.835 5 +/- 0.537 0 and 3.839 6 +/- 0.223 0, respectively, indicating there were significant differences between two groups (P < 0.05).

CONCLUSION

XLGB can obviously promote the femoral fracture healing in OVX rats, and speed the transformation of woven bone into lamellar bone, which may rely on its role of enhancing expression of BMP-2 and VEGF.

目的

评价仙灵骨葆（XLGB）对去卵巢（OVX）大鼠股骨骨折愈合的影响。

方法

将40只12周龄、体重（258±14）g的雌性SD大鼠随机分为4组（每组n = 10）：A组，打开腹腔行假手术；B组，双侧卵巢切除术；C组，双侧卵巢切除术后，右股骨干中段横行骨折并髓内钉固定，给予生理盐水灌胃；D组，双侧卵巢切除术后，右股骨干中段横行骨折并髓内钉固定，给予250 mg/（kg·d）XLGB灌胃。分别于术后0、1、2、3、4和5周测量A、B组大鼠体重。术后5周获取每组大鼠右侧股骨。采用双能X线吸收法测量全股骨骨密度（tBMD）、股骨远端骨密度（dBMD）和股骨中段骨密度（mBMD），对C、D组进行CR摄片、HE染色及免疫组织化学染色。

结果

术后3、4和5周时，B组大鼠体重明显高于A组（P < 0.05），表明绝经后骨质疏松动物模型成功建立。CR片显示，D组骨痂较多，骨折线模糊，而C组骨痂较少，骨折线清晰。B组的tBMD和dBMD远低于A组，D组的mBMD显著高于C组（P < 0.05），D组的tBMD和dBMD高于C组，但两组间差异无统计学意义（P > 0.05）。组织学观察显示，与C组相比，D组多数骨折端达到骨愈合，骨髓腔内可见明显的毛细血管长入。免疫组织化学染色显示，C、D组骨形态发生蛋白-2（BMP-2）的积分吸光度（IA）值分别为2.236 6±0.181 8和3.727 3±0.874 2，血管内皮生长因子（VEGF）的IA值分别为2.835 5±0.537 0和3.839 6±0.223 0，两组间差异有统计学意义（P < 0.05）。